A Fluorimetric Method for Measurement of Erythrocyte Transketolase Activity

Correspondence: Measurement of erythrocyte transketolase activity

CorreSpondence Measurement of erythrocyte transketolase activity Correspondence from Donald E. King C. R. Milner et al.'s recent paper compared automated versus manual procedures for the 'Measurement of erythrocyte trans-ketolase activity on a discrete analyser' (Journal of Automatic Chemistry, Vol. 4, No. 4, pp. 183-185). In it the author uses regression analysis to evaluate the similarity of ...

The measurement of erythrocyte transketolase activity on a discrete analyser

Transketolase (TK, EC.2.2.1.1) is a thiamine-dependent enzyme which is widely used for assessing thiamine deficiency. The TK activity in erythrocytes is usually decreased during thiamine deficiency and this activity can be enhanced in vitro by the addition of thiamine pyrophosphate (TPP). The relative increase in enzyme activity, expressed as a percentage, is called the TPP effect. The TK kinet...

Erythrocyte transketolase activity measured on the Gemstar discrete analyser

Erythrocyte transketolase activity in patients with diabetic and alcoholic neuropathies.

INTRODUCTION The activity of erythrocyte transketolase induced by thiamine pyrophosphate and normalized to age of the patient is a marker of thiamine metabolism disturbances with pathological consequences in the central and peripheral nervous system. The measurement of erythrocyte transketolase activity enables evaluation of the thiamine status and therapeutic decisions in the disorders of the ...

A fluorimetric method for red blood cell sorbitol dehydrogenase activity.

A new fluorimetric method for the quantification of red blood cell (RBC) sorbitol dehydrogenase is described. It is based on the oxidation of sorbitol to fructose, in presence of NAD+, catalysed by the RBC-sorbitol dehydrogenase. The quantity of NADH formed is then measured in a filter fluorimeter. Comparison with an indirect spectrophotometric assay yielded good correlation; however, the prese...